Electrophoretic Behavior of Dyestuff-Containing Liposome in a Capillary with Original On-Line Chemiluminescence Detection.

  • TSUKAGOSHI Kazuhiko
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • OKUMURA Yasuo
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • AKASAKA Hideshi
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • NAKAJIMA Riichiro
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
  • HARA Tadashi
    Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University

書誌事項

タイトル別名
  • Electrophoretic Behavior of Dyestuff-Co

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抄録

An Eosin Y-containing liposome solution as a sample was for the first time migrated and detected by capillary zone electrophoresis with chemiluminescence detection using a peroxyoxalate system. Two peaks, as a typical example, were recorded on the electropherogram: the first one was due to Eosin Y entrapped in liposome; the second one was due to free Eosin Y in the bulk solution. The electropherogram, comprising two peaks, readily, rapidly, and sensitively offered various forms of information about the liposome, including the preparation course, stability, permeability, and surface charge. Furthermore, an Eosin Y-containing liposome solution gave a good linear relationship between the concentration and chemiluminescence intensity. Only the chemiluminescence detection was effective for examining the electrophoretic behavior of the Eosin Y-containing liposome in a capillary. Spectrophotometric and fluorescent detectors could not be utilized for detecting the liposome, due mainly to the low sensitivities.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 12 (6), 869-874, 1996

    社団法人 日本分析化学会

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