Electrophoretic Behavior of Dyestuff-Containing Liposome in a Capillary with Original On-Line Chemiluminescence Detection.
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- TSUKAGOSHI Kazuhiko
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
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- OKUMURA Yasuo
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
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- AKASAKA Hideshi
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
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- NAKAJIMA Riichiro
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
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- HARA Tadashi
- Department of Chemical Engineering and Materials Science, Faculty of Engineering, Doshisha University
書誌事項
- タイトル別名
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- Electrophoretic Behavior of Dyestuff-Co
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抄録
An Eosin Y-containing liposome solution as a sample was for the first time migrated and detected by capillary zone electrophoresis with chemiluminescence detection using a peroxyoxalate system. Two peaks, as a typical example, were recorded on the electropherogram: the first one was due to Eosin Y entrapped in liposome; the second one was due to free Eosin Y in the bulk solution. The electropherogram, comprising two peaks, readily, rapidly, and sensitively offered various forms of information about the liposome, including the preparation course, stability, permeability, and surface charge. Furthermore, an Eosin Y-containing liposome solution gave a good linear relationship between the concentration and chemiluminescence intensity. Only the chemiluminescence detection was effective for examining the electrophoretic behavior of the Eosin Y-containing liposome in a capillary. Spectrophotometric and fluorescent detectors could not be utilized for detecting the liposome, due mainly to the low sensitivities.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 12 (6), 869-874, 1996
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679232410496
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- NII論文ID
- 130003528108
- 10002409914
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK28XnsFChur4%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4099080
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可