Simultaneous Determination of Menaquinone-4 and Its Metabolite in Human Osteoblasts by High-Performance Liquid Chromatography/Atmospheric Pressure Chemical Ionization Tandem Mass Spectrometry.
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- SANO Yoshihisa
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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- KIKUCHI Kiyomi
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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- TADANO Kyoichi
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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- HOSHI Kazuko
- Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontolog
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- KOSHIHARA Yasuko
- Department of Biosignal Research, Tokyo Metropolitan Institute of Gerontolog
Bibliographic Information
- Other Title
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- Simultaneous Determination of Menaquino
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Abstract
A simple and sensitive method is described for the determination of menaquinone-4 and its metabolite menaquinone-4 epoxide in human periosteal osteoblasts. The method is based on high-performance liquid chromatography/ atmospheric pressure chemical ionization mass spectrometry, using deuterated menaquinone-4 as an internal standard. Selected reaction monitoring (SRM) is employed for selectivity and sensitivity. This enables quantification in a short period of time (within 5min) over the extended ranges 1-1000ng/ml for menaquinone-4 and 2.5-5000ng/ml for menaquinone-4 epoxide with acceptable precision and accuracy. The method was used to determine both compounds in human osteoblasts, following incubation with menaquinone-4. It is suggested that menaquinone-4 is metabolized to menaquinone-4 epoxide in bone as well as in liver, indicating the drug has the same role in γ-carboxylation of vitamin K dependent proteins in both tissues. It is expected that this method will contribute to elucidating the pharmacological mechanism of action of menaquinone-4 on bone metabolism.
Journal
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- Analytical Sciences
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Analytical Sciences 13 (1), 67-73, 1997
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390001204258465920
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- NII Article ID
- 130003528137
- 10002410551
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK2sXht1KjtL0%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4142759
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- Web Site
- https://ndlsearch.ndl.go.jp/books/R000000004-I4142759
- https://link.springer.com/content/pdf/10.2116/analsci.13.67.pdf
- https://link.springer.com/article/10.2116/analsci.13.67/fulltext.html
- http://www.jstage.jst.go.jp/article/analsci1985/13/1/13_1_67/_pdf
- https://search.jamas.or.jp/link/ui/1997104582
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
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- CiNii Articles
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- Abstract License Flag
- Disallowed