Sequential Analysis of Reducing Oligosaccharides Based on a Chemical Reaction Using 8-Amino-2-naphthalenesulfonic Acid.

• HONG Seon-Pyo

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University Tokyo

• SANO Akira

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University Tokyo

• NAKAMURA Hiroshi

  Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Science University Tokyo

書誌事項

タイトル別名

• Sequential Analysis of Reducing Oligosa

抄録

A method for the sequential analysis of reducing sugars has been developed based on repetition of the following procedures: 1) derivatization of a reducing oligosaccharide with 8-amino-2-naphthalenesulfonic acid (ANS) to produce a Schiff base, 2) reduction of the base with sodium cyanoborohydride, 3) methoxycarbonylation of the secondary amino group, 4) cleavage of the glycoside bond next to the reducing end, through intramolecular acid hydrolysis by the action of a sulfonic acid group of the ANS derivative, 5) identification of the liberated reducing end by high-performance liquid chromatography (HPLC), and finally 6) recovery of the resultant oligosaccharide fragment from the cleavage reaction mixture. A satisfactory yield in the cleavage reaction and a quantitative recovery of the oligosaccharide fragments were obtained when the cleavage reaction of the ANS derivative was carried out at 140°C for 30min in dioxane-dimethyl sulfoxide (7:3). The method was successfully applied to the sequential analysis of some reducing di-and trisaccharides.

収録刊行物

• Analytical Sciences

  Analytical Sciences 13 (3), 473-478, 1997

  社団法人 日本分析化学会

キーワード

• Sequential analysis
• reducing oligosaccharide
• 8-amino-2-naphthalenesulfonic acid
• high-performance liquid chromatography
• intramolecular acid hydrolysis

詳細情報

• CRID: 1390282679229177856
• NII論文ID: 130003416637
• NII論文ID: 10002411859
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.13.473
• COI: 1:CAS:528:DyaK2sXjvFWhtL4%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 4231301
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4231301
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.13.473.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.13.473/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/13/3/13_3_473/_pdf
• 本文言語コード: en
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