Poly(N-isopropylacrylamide) Carrying Double-Stranded DNA for Affinity Separation of Genotoxins.

• UMENO Daisuke

  Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University

• MAEDA Mizuo

  Department of Chemical Science and Technology, Faculty of Engineering, Kyushu University

Bibliographic Information

Other Title

• Poly N isopropylacrylamide Carrying Dou
• Poly(N-isopropylacrylamide) Carrying for Affinity Separation of Genotoxins Double-Stranded DNA

Abstract

We immobilized double-stranded DNA on poly(N-isopropylacrylamide), which is temperature-sensitive and gives precipitates from an aqueous solution when heated over 31°C. Photochemical conjugation with a vinyl derivative of psoralen was demonstrated to make the DNA polymerizable with a vinyl monomer. The radical copolymerization with N-isopropylacrylamide gave the temperature-responsive conjugate. The conjugate was shown to capture ethidium, a D0NA-binding genotoxin, and precipitate with it when heated. About 95% of ethidium was separated from its highly diluted solution (3ppm).

Journal

• Analytical Sciences

  Analytical Sciences 13 (4), 553-556, 1997

  The Japan Society for Analytical Chemistry

Keywords

• DNA
• psoralen
• poly(<i>N</i>-isopropylacrylamide)
• ethidium
• precipitation
• affinity separation
• genotoxin

Details

• CRID: 1390001204258584704
• NII Article ID: 130003528201
• NII Article ID: 10002412118
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.13.553
• COI: 1:CAS:528:DyaK2sXlsVClt7g%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 4277864
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4277864
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.13.553.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.13.553/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci1985/13/4/13_4_553/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed