Simultaneous Determination of 3, 4-Dihydroxyphenylglycol, 4-Hydroxy-3-methoxymandelic Acid, 4-Hydroxy-3-methoxyphenylglycol and Their Precursors in Rat-Brain Tissue by High-Performance Liquid Chromatography with Electrochemical Detection

この論文にアクセスする

この論文をさがす

著者

抄録

A method was developed for the quantification of 3, 4-dihydroxyphenylglycol (DHPG), 4-hydroxy-3-methoxymandelic acid (VMA), 4-hydroxy-3-methoxyphenylglycol (MHPG). The separation and determination of these compounds in biological samples were previously thought to be very difficult. In this work the separation has been well achieved by high-performance liquid chromatography on a reversed-phase column using three mobile phases with a step-wise gradient elution mode. The condition for coulometric detection has been developed for an effective determination of these compounds. In the analysis of the rat-brain tissue, DHPG, VMA and MHPG were separated and determined successfully. The average levels of DHPG, VMA, and MHPG in nine different rat-brain tissue samples were 11.8±0.57, 2.13±0.13 and 6.77±1.84ng/g wet brain, respectively. Simultaneously, their precursors DOPA, DA, NE, DOPAC, HVA, 3MT and NMN, and indolamine 5HT and its metabolite 5HIAA may also be determined by the same chromatographic run.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 13(4), 583-587, 1997-08-10 

    The Japan Society for Analytical Chemistry

参考文献:  18件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10002412193
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4277869
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ