Stripping Voltammetric Determination of Choline Based on Micro-Fabricated Composite Membrane

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A novel stripping voltammetric method for the determination of non-redox ionic species is presented. Using choline ion (Ch+) as an example, we demonstrate how a micro machined PVC-NPOE gel membrane can be used to accumulate Ch+ ion under potentiostatic steady-state conditions. The amperometric stripping of the target ion can be carried out by any voltammetric method but we presently illustrate the principle using linear sweep voltammetry. The effect of accumulation time, accumulation potential and choline ion concentration has been examined. The present work also includes the quantitative study of an enzyme reaction generating choline, namely the reaction of calcium phosphocholine with alkaline phosphatase. With this experimental approach, µg ml-1 concentration level of alkaline phosphatase enzyme can be determined.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 14(1), 71-78, 1998-02-10 

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10002414122
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4406425
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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