Paint-Freeze Method to Form Self-Assembled Alkanethiol/Phospholipid Bilayers on Gold
A paint-freeze method for preparing self-assembled alkanethiol/phospholipid bilayers on a gold surface has been described (by cyclic voltammetry, a.c impedance, polarized FTIR-ATR) to be well-ordered and packed, stable, solvent-free bilayers. The lipid order parameter was 0.67, calculated from the dichroic ratio, consistent with a well-ordered lipid film in which the methylene groups have segmental flexibility and are disordered to a degree which is typical for a lipid bilayer in the liquid-crystalline phase. Such a supported membrane provides a useful way for studies in biophysics, physiology and electrochemistry.
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 14(1), 117-120, 1998-02-10