Single Molecule Detection by Laser Two-Photon Excited Fluorescence in a Capillary Flowing Cell
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The two-photon excited fluorescence of fluorescein and Rhodamine B in a flowing sample cell was observed using a self mode-locked Ti-sapphire laser (180 fs pulse). The detection limit of fluorescein was less than 2 molecules in the probe volume. The single molecule detection of Rhodamine was successfully performed in a flowing cell, and the number of photon bursts agreed approximately with that expected.
- Analytical Sciences
Analytical Sciences 14(5), 913-916, 1998-10-10
The Japan Society for Analytical Chemistry