Polymer-Mediated Extraction for Microplate Reader Spectrophotometric Determination of ppb-Level Copper(II) Ion in Water.
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- SAITOH Tohru
- School of Life Science, Tokyo University of Pharmacy and Life Science
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- HAGA Masumi
- School of Life Science, Tokyo University of Pharmacy and Life Science
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- SAKURAI Teruaki
- School of Life Science, Tokyo University of Pharmacy and Life Science
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- KAISE Toshikazu
- School of Life Science, Tokyo University of Pharmacy and Life Science
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- MATSUBARA Chiyo
- School of Life Science, Tokyo University of Pharmacy and Life Science
Bibliographic Information
- Other Title
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- Polymer-Mediated Extraction for Micropl
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Abstract
A negatively charged metal chelate, bis(bathocuproinedisulfonic acid)-copper(I), was formed and stably solubilized in an aqueous solution of a water-soluble polymer, poly(N-isopropylacrylamide) (PNIPAAm), at room temperature. When the solution was warmed at 40°C, PNIPAAm became water-insoluble. It then formed a gum-like aggregate (polymer phase) having a very small volume. The chelate was difficult to be extracted to the polymer phase in the absence of counter ions, but was efficiently incorporated when appropriate amounts of quaternary ammonium ion are present as the counter ions. The recovery of the chelate increased with increasing the concentration or the hydrophobic properties of the counter ions, indicating the ion-pair extraction of the charged chelate into the polymer phase. In the presence of 1 mM dodecyltrimethylammonium chloride, the recovery of the chelate was quantitative. After the polymer phase was dissolved by 1-propanol in the hole of a 96-hole microplate, the absorbance at 450 nm of copper(I) chelate was measured. A slightly curved calibration was obtained in the copper(II) concentration range from 5×10-9 M to 5×10-7 M. The standard deviation was 6% (n=5) for 5×10-8 M copper(II) ions.
Journal
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- Analytical Sciences
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Analytical Sciences 14 (5), 929-933, 1998
The Japan Society for Analytical Chemistry
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Details
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- CRID
- 1390001204258328320
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- NII Article ID
- 10002417267
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1cXmslWqurc%3D
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- ISSN
- 13482246
- 09106340
- http://id.crossref.org/issn/09106340
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- NDL BIB ID
- 4576990
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed