Simultaneous Catalyzing of Uricase- and Catalase-Like Reactions of Resins Modified with Metalloporphyrins and Its Application for Determination of Uric Acid^1
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Some anion-exchange resins modified with water-soluble metalloporphyrins exhibited an ability to catalyze simultane-ously both uricase- and catalase-like reactions. The resin modified with manganese-tetrakis(4-sulfophenyl)porphine (Mn-TPPSr), which showed the highest activity among the resins tested, was useful for the indirect determination of uric acid by measuring the amount of formaldehyde produced. The calibration curve was linear over the range from 10 μg to 200 μg of uric acid with a relative standard deviation of 1.8% (n=10) for 200 μg uric acid. Mn-TPPSr was also applied for the determination of uric acid in sera.
- Analytical Sciences
Analytical Sciences 14(5), 935-939, 1998-10-10
The Japan Society for Analytical Chemistry