Microfabricated On-Line Sensor for Continuous Monitoring of L-Glutamate

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抄録

An on-line sensor was developed by using a micro-machining technique for the continuous measurement of extracellular glutamate. The sensor was made by bonding two glass plates together. One of the plates has a rectangular flow channel which is connected to both a sampling and an outlet capillary. The other has a carbon film-based electrochemical cell, which consists of an enzyme-modified electrode surrounded by polymer film to form a thin-layer-channel flow cell. The sensor has a high analyte conversion efficiency due to the cell structure. As a result, we obtained a low detection limit of 44 nM (S/N=3). The cathodic current started to increase about 10 s after sample introduction at a flow rate of 2 μl/min due to its small inner volume. Using the sensor, we can monitor the extracellular <font size=-2>L</font>-glutamate increase caused by stimulating it with KCl and γ-aminobutyric acid (GABA) by locating the sampling capillary very close to the cell.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 14(5), 947-953, 1998-10-10 

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10002417312
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4576993
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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