Microfabricated On-Line Sensor for Continuous Monitoring of L-Glutamate.
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- NIWA Osamu
- NTT Basic Research Laboratories
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- HORIUCHI Tsutomu
- NTT Basic Research Laboratories
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- KURITA Ryoji
- NTT Advanced Technology
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- TABEI Hisao
- NTT Advanced Technology
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- TORIMITSU Keiichi
- NTT Basic Research Laboratories
Bibliographic Information
- Other Title
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- Microfabricated On-Line Sensor for Cont
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Abstract
An on-line sensor was developed by using a micro-machining technique for the continuous measurement of extracellular glutamate. The sensor was made by bonding two glass plates together. One of the plates has a rectangular flow channel which is connected to both a sampling and an outlet capillary. The other has a carbon film-based electrochemical cell, which consists of an enzyme-modified electrode surrounded by polymer film to form a thin-layer-channel flow cell. The sensor has a high analyte conversion efficiency due to the cell structure. As a result, we obtained a low detection limit of 44 nM (S/N=3). The cathodic current started to increase about 10 s after sample introduction at a flow rate of 2 μl/min due to its small inner volume. Using the sensor, we can monitor the extracellular L-glutamate increase caused by stimulating it with KCl and γ-aminobutyric acid (GABA) by locating the sampling capillary very close to the cell.
Journal
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- Analytical Sciences
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Analytical Sciences 14 (5), 947-953, 1998
The Japan Society for Analytical Chemistry
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Details 詳細情報について
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- CRID
- 1390282679235042560
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- NII Article ID
- 10002417312
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- NII Book ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1cXmslWqu7w%3D
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- ISSN
- 13482246
- 09106340
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- NDL BIB ID
- 4576993
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed