High-Performance Liquid Chromatographic Determination of Oxalic Acid in Tea Using Tris(1, 10-phenanthroline)-ruthenium(II) Chemiluminescence
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The chemiluminescence (CL) of Ru(phen)32+ was applied to HPLC determination of oxalic acid, which was separated by a C18 reverse-phase column with a mobile phase of 0.02 mol/l NH4Ac, mixed with 0.25 mol/l Ru(phen)32+ and 2.0 mmol/l Ce(SO4)2 (0.08 mol/l H2SO4), and then passed through a homemade CL detector. The reaction of Ce(IV) oxidized Ru(phen)32+ and the oxalic acid emitted light. The detection limit was 6.2×10-6 mol/l for oxalic acid at a S/N ratio of 3, the relative standard deviation for 5 replicate injections of 1×10-3 mol/l oxalic acid standard was calculated as 5.6%, and the lin-ear calibration range was 1×10-5 to 4×10-3 mol/l. The method was successfully applied to determination of oxalic acid in tea.
- Analytical Sciences
Analytical Sciences 14(5), 971-973, 1998-10-10
The Japan Society for Analytical Chemistry