Time-Resolved Fluorometric Deterction of DNA Using a Tetradentate β-Diketonate Europium Chelate as a Label
A tetradentate β-diketonate europium chelate, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chloro-sulfo-o-terphenyl(BHHCT)-Eu3+, was labeled to a streptavidin-bovine serum albumin conjugate for time-resolved fluorometric detection of λDNA by hybridization with a biotinylated probe DNA in a microtiter well. The method gave a detection limit of 23 pg/well (solid-phase measurement) and 4 pg/well (solution-phase measurement) for specific DNA.
- Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 15(2), 121-124, 1999-02