Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate .BETA.-Diketonate Europium Chelate as a Label.

• YUAN Jingli

  Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)

• WANG Guilan

  Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)

• KIMURA Hiroko

  Japan Science and Technology Corporation (JST) Department of Forensic Medicine, Juntendo University School of Medicine

• MATSUMOTO Kazuko

  Department of Chemistry, Waseda University Japan Science and Technology Corporation (JST)

Bibliographic Information

Other Title

• Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate β-Diketonate Europium Chelate as a Label
• Highly Sensitive Detection of Bensulfuron-methyl by Time-Resolved Fluoroimmunoassay Using a Tetradentate ベーターDiketonate Europium Chelate as a Label

Abstract

A tetradentate β-diketonate europium fluorescent chelate, 4,4′-bis(1″,1″,1″,2″,2″,3″,3″-heptafluoro-4″,6″-hexanedion-6″-yl)-chlorosulfo-o-terphenyl (BHHCT)-Eu³⁺, was used as a label for highly sensitive time-resolved fluoroimmunoassay of bensulfuron-methyl (BSM). A BSM-BSA (BSA=bovine serum albumin) conjugate was labeled with BHHCT-Eu³⁺ and the labeled BSM-BSA conjugate was used for competitive immunoassay. The assay was carried out in a 96-well microtiter plate coated with the anti-BSM monoclonal antibody. The method gives the detection limit of 24 pg/ml, which is one to two orders of magnitude lower than those of the conventional HPLC and other methods. The RSD of the present method is less than 10%, and the recovery is in the range of 90 - 110% for BSM water samples.

Journal

• Analytical Sciences

  Analytical Sciences 15 (2), 125-128, 1999

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204257841152
• NII Article ID: 10002418608
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.15.125
• COI: 1:CAS:528:DyaK1MXhslansL8%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 4648251
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4648251
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.15.125.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.15.125/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/15/2/15_2_125/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed