Flow-Injection Spectrophotometric Determination of Nitrite and Nitrate Biological Samples

この論文にアクセスする

この論文をさがす

著者

抄録

Nitrite and nitrate in biological samples can act as an indicator of nitric oxide generated in vivo and have attracted a lot of attention as a messenger of diverse physiological processes. For the determination of such nitrogen oxides, a simple and sensitive flow-injection method coupled with a successful pretreatment method was developed. Nitrite and nitrate in sample solution were injected into a carrier solution containing EDTA and ammonium buffer, and flowed into the copperized cadmium (Cd/Cu) reduction column installed on-line. Here the nitrate was reduced to nitrite, and then nitrite was determined spectrophotometrically on the basis of a diazotization/coupling reaction. The detection limits for nitrite and nitrate were 5×10-8 M, and the sampling rate was about 40 samples per hour for nitrate determination. The biological samples, such as serum, plasma and cell culture medium, were deproteinized using a batchwise NaOH-ZnSO4 method before FIA measurement. The NaOH-ZnSO4 deproteinization method was very effective in term of the recovery of nitrate and the maintenance of the reduction efficiency of the Cd/Cu column. The relative standard deviations were 2.1 - 3.1%, and the recoveries were 95 - 100% for the determination of nitrite and nitrate in serum samples through the whole procedure. In the case of cell culture medium, the proposed deproteinization was especially effective for removing the interfering amino acids containing sulfur atom by using the reaction between Zn2+ in deproteinization agent and sulfur atom in analytical substances.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry  

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 15(2), 129-134, 1999-02 

    社団法人 日本分析化学会

参考文献:  17件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  4件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10002418630
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4648255
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ