Potentiometric Determination of NAD(P)H Coenzymes Using a Nitrite-Selective Membrane Electrode and a Nitrate Reductase Enzyme and the Application to Glucose Assay in Human Serum

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NAD(P)H coenzyme concentrations were determined with the combination of a nitrite-selective membrane electrode and a nitrate reductase enzyme. The method was based on the detection of nitrite formed by the enzymatic reaction of NAD(P)H with nitrate catalyzed by nitrate reductase. The lower detection limits for both these coenzymes were around 100 µM. This system was applied to detect glucose by combining an additional glucose dehydrogenase enzyme. Serum glucose levels could be determined through the detection of nitrite.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 15(2), 135-139, 1999-02

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10002418648
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    4648316
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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