Buffer Effect on the Photoelectrochemical Response of Bacteriorhodopsin.
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- SAGA Yoshitaka
- Institute of Industrial Science, University of Tokyo
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- WATANABE Tadashi
- Institute of Industrial Science, University of Tokyo
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- KOYAMA Koichi
- Ashigara Research Laboratories, Fuji Photo Film Co., Ltd.
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- MIYASAKA Tsutomu
- Ashigara Research Laboratories, Fuji Photo Film Co., Ltd.
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The photoelectrochemical response of bacteriorhodopsin (bR) was studied in buffered electrolyte solutions. The photocurrent intensity was inversely proportional to the buffer concentration in a wide range, and the response time became shorter with increasing buffer concentration. When plotted as a function of the electrolyte pH, the ratio of the peak photocurrent in the presence of a buffer to that in the absence of the buffer exhibited a minimum at a pH nearly equal to the pK′a of the buffer. These results are explained by invoking the buffering capacity, and are in line with the view that the transient bR photoresponse originates in the pH change near the electrode due to proton release/uptake by bR molecules.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 15 (4), 365-369, 1999
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390282679232049920
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- NII論文ID
- 10002419420
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- NII書誌ID
- AA10500785
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- COI
- 1:CAS:528:DyaK1MXisVSntLg%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 4712445
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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