Antibodies with the Cell-type Specificity to the Morula Cells of the Solitary Ascidians Styela Rustica and Boltenia Echinata

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The separation of the blood cells of <i>Styela rustica</i> (<i>Styelidae</i>, <i>Stolidobranchiae</i>) in discontinuous Percoll gradient showed 4 fractions. The 4th and bottom most fraction contained 90-100% of morula cells. The protein composition of the morula cell fraction revealed on SDS-PAGE showed two major proteins with m.w. 47 and 26 kDa. These proteins were heavily positively charged. Polyclonal antiserums against these proteins were raised. Each antiserum reacted with both proteins only in morula cells on the blot after SDS-PAGE and stained the proper protein without crossreaction on the blot after AU-PAGE. The only type of cells stained with antibodies in circulating blood, in the tunic and on the tunic wound surface in paraffin sections of another species <i>Boltenia echinata</i> (<i>Pyuridae</i>, <i>Stolidobranchiae</i>) were morula cells. The morula-type specific antibodies obtained recognized major positively charged proteins which were apparently structural substrates for the phenoloxidase tanning.

収録刊行物

  • Cell structure and function  

    Cell structure and function 23(6), 349-355, 1998-12-01 

    Japan Society for Cell Biology

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各種コード

  • NII論文ID(NAID)
    10002420340
  • NII書誌ID(NCID)
    AA0060007X
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03867196
  • NDL 記事登録ID
    4639055
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-V38
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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