Disruption of Circumferential Actin Filament Causes Disappearance of Occludin from the Cell Borders of Rat Hepatocytes in Primary Culture without Distinct Changes of Tight Junction Strands.
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- Kojima Takashi
- Department of Pathology, Cancer Research Institute
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- Sawada Norimasa
- Department of Pathology, Sapporo Medical University School of Medicine, Sapporo 060, Japan
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- Yamamoto Masao
- Department of Anatomy, Hiroshima University School of Medicine, Hiroshima 734, Japan
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- Kokai Yasuo
- Department of Pathology, Sapporo Medical University School of Medicine
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- Mori Michio
- Department of Pathology, Sapporo Medical University School of Medicine
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- Mochizuki Yohichi
- Department of Pathology, Cancer Research Institute
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抄録
We investigated the relationship of actin filament organization to occludin and tight junction strands in primary cultured rat hepatocytes using an actin depolymerizing agent, mycalolide B. In control cultures, well-developed circumferential actin filaments and occludin immunoreactivity were observed on the most subapical plasma membrane of the cells, and tight junction strands formed well-developed networks in freeze-fracture replicas. In hepatocytes treated with 3 μM mycalolide B for 6h, circumferential actin filaments and occludin immunoreactivity disappeared from the cell borders. However, there were no marked abnormalities of tight junction strands in freeze fracture replicas. Similar results were obtained from cells cultured in medium with 0.05 mM Ca2+ for 6h. The close association of occludin with actin and the existence of intact tight junction strands that are virtually free of both occludin and actin suggest a physiological role of occludin, but not the other proteins forming the tight junction strands, in the linkage between actin cytoskeleton and tight junction.
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 24 (1), 11-17, 1999
日本細胞生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679671485184
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- NII論文ID
- 130004137283
- 30023110019
- 10002420518
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- NII書誌ID
- AA0060007X
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- COI
- 1:CAS:528:DyaK1MXisVagtLc%3D
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- ISSN
- 13473700
- 03867196
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- NDL書誌ID
- 4685321
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- PubMed
- 10355874
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可