The Effects of Various GTP Analogues on Microtubule Assembly.

• Muraoka Masako

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Fukuzawa Hiromi

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Nishida Akiko

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Okano Kyoko

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Tsuchihara Tomoko

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Shimoda Asako

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Suzuki Yuko

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Sato Mamiko

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Osumi Masako

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

• Sakai Hikoichi

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan

書誌事項

タイトル別名

• Effects of Various GTP Analogues on Microtubule Assembly

抄録

We synthesized 27 GTP analogues with modification or substitution at positions C2, C6, C8 and ribose moiety to investigate their effect on microtubule (Mt) assembly. It was found that C2 and C6 are both functional for the analogues supporting Mt assembly. It was surprising to find that 2-amino-ATP (n2ATP) substantially supports assembly, and that the appearance of the assembled Mts was indistinguishable from those assembled in the standard GTP assembly buffer solution. Furthermore, 2-amino dATP and dGTP are even more potent than GTP in supporting assembly. The substitution of oxo group at C6 with reactive thiol largely reduced the activity of the analogue to support assembly. When free rotation of the glycosidic linkage of GTP was blocked by the introduction of sulfur atom between C8 and C2’ of ribose moiety, it resulted in total suppression of assembly. Purine nucleoside triphosphate was found to support assembly better than GTP, and even more efficient was 2-amino purine nucleoside triphosphate. Interestingly, their deoxy-type analogues were totally inhibitory. Although 2-amino 8-hydroxy ATP and other analogues supported assembly much better than did GTP, their diphosphate analogues were totally incapable of supporting assembly. Finally, bulky fluorescent probes were introduced at C3’ of ribose moiety (Mant-8-Br-GTP or Mant-GTP) to visualize the fluorescent signal in assembled Mts. Even in this case, the number of most protofilaments was found to be 14, consistent with that found in Mts assembled in GTP standard buffer solution.

収録刊行物

• Cell Structure and Function

  Cell Structure and Function 24 (2), 101-109, 1999

  日本細胞生物学会

キーワード

• tubulin
• microtubules
• GTP analogues
• microtubule assembly

詳細情報

• CRID: 1390282679671379456
• NII論文ID: 130004137292
• NII論文ID: 10002420876
• NII書誌ID: AA0060007X
• DOI: 10.1247/csf.24.101
• COI: 1:CAS:528:DyaK1MXjs1ejsrc%3D
• ISSN: 13473700
• ISSN: 03867196
• NDL書誌ID: 4728535
• PubMed: 10362073
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4728535
• Web Site: http://www.jstage.jst.go.jp/article/csf/24/2/24_2_101/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可