泡沫分離法によるタンパク質の分離濃縮 Separation and Recovery of Protein fron Dilute Solution by Foam Fractionation

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From the point of view of the protection of our circumstances and recycling of materials, it is important to separate and recover proteins economically and in an industrial scale . Protein is easily affected by heat, pH, solvent and so on, by which it changes the chemical properties. In this work, the effect of several factors on the separation and recovery of protein, especially albumin and hemoglobin was investigated using a foam fractionationmethod. Also we measured the stability of the froth and foaming ability of protein solution . We tried to express them quantitatively into the grade. At the low flow rate of gas, both proteins can be separated from the solution at high percentage . Also, the highest recovery was obtained at pH of the point of zero charge. It is another way to add some salts in the solution which have influence to the improvement of the recovery. Compared with the life span of the froth between albumin and hemoglobin, albumin is superior to hemoglobin. The life of the froth of albumin is considerably affected by pH value of the solution. On the contrary, that of hemoglobin strongly depends on the concentration in stead of pH. We can get the high concentration ratio only by using the higher froth layer,namely the higher column length.We achieved a selective separation between albumin and hemoglobin at the concentration ratio of 10:1 at the pH of point of zero charge of albumin; moreover by using a surface active agent, e.g. Tween 20, the separation ratio was improved up to 43: 1.http://ci.nii.ac.jp/naid/10002470838

From the point of view of the protection of our circumstances and recycling of materials, it is important to separate and recover proteins economically and in an industrial scale. Protein is easily affected by heat, pH, solvent and so on, by which it changes the chemical properties. In this work, the effect of several factors on the separation and recovery of protein, especially albumin and hemoglobin was investigated using a foam fractionation method. Also we measured the stability of the froth and foaming ability of protein solution. We tried to express them quantitatively into the grade.<BR>At the low flow rate of gas, both proteins can be separated from the solution at high percentage. Also, the highest recovery was obtained at pH of the point of zero charge. It is another way to add some salts in the solution which have influence to the improvement of the recovery. Compared with the life span of the froth between albumin and hemoglobin, albumin is superior to hemoglobin. The life of the froth of albumin is considerably affected by pH value of the solution. On the contrary, that of hemoglobin strongly depends on the concentration in stead of pH. We can get the high concentration ratio only by using the higher froth layer, namely the higher column length.<BR>We achieved a selective separation between albumin and hemoglobin at the concentration ratio of 10: 1 at the pH of point of zero charge of albumin; moreover by using a surface active agent, e.g. Tween 20, the separation ratio was improved up to 43: 1.

収録刊行物

  • 資源処理技術 : 浮選 = Resources processing  

    資源処理技術 : 浮選 = Resources processing 44(2), 63-68, 1997-08-05 

    資源処理学会

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各種コード

  • NII論文ID(NAID)
    10002470838
  • NII書誌ID(NCID)
    AN10136265
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    09124764
  • NDL 記事登録ID
    4267855
  • NDL 雑誌分類
    ZP41(科学技術--金属工学・鉱山工学)
  • NDL 請求記号
    Z17-275
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
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