Separation and Recovery of Protein from Dilute Solution by Foam Fractionation.

• MATSUMOTO Shigeno

  関西大学工学部化学工学科

• OGAWA Kazuyuki

  東洋ゴム工業株式会社

• SHIBATA Junji

  関西大学工学部化学工学科

Bibliographic Information

Other Title

• 泡まつ分離法によるタンパク質の分離濃縮
• 泡沫分離法によるタンパク質の分離濃縮
• ホウマツ ブンリホウ ニヨル タンパクシツ ノ ブンリ ノウシュク

Abstract

From the point of view of the protection of our circumstances and recycling of materials, it is important to separate and recover proteins economically and in an industrial scale. Protein is easily affected by heat, pH, solvent and so on, by which it changes the chemical properties. In this work, the effect of several factors on the separation and recovery of protein, especially albumin and hemoglobin was investigated using a foam fractionation method. Also we measured the stability of the froth and foaming ability of protein solution. We tried to express them quantitatively into the grade.<BR>At the low flow rate of gas, both proteins can be separated from the solution at high percentage. Also, the highest recovery was obtained at pH of the point of zero charge. It is another way to add some salts in the solution which have influence to the improvement of the recovery. Compared with the life span of the froth between albumin and hemoglobin, albumin is superior to hemoglobin. The life of the froth of albumin is considerably affected by pH value of the solution. On the contrary, that of hemoglobin strongly depends on the concentration in stead of pH. We can get the high concentration ratio only by using the higher froth layer, namely the higher column length.<BR>We achieved a selective separation between albumin and hemoglobin at the concentration ratio of 10: 1 at the pH of point of zero charge of albumin; moreover by using a surface active agent, e.g. Tween 20, the separation ratio was improved up to 43: 1.

Journal

• RESOURCES PROCESSING

  RESOURCES PROCESSING 44 (2), 63-68, 1997

  The Resources Processing Society of Japan

Details

• CRID: 1390001204267331456
• NII Article ID: 10002470838
• NII Book ID: AA11907992
• DOI: 10.4144/rpsj1986.44.63
• ISSN: 18839150
• ISSN: 09124764
• HANDLE: 10112/5501
• NDL BIB ID: 4267855
• Web Site: https://kansai-u.repo.nii.ac.jp/records/11375
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4267855
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed