Control of the Cross Membrane Potential of a Cultured Tobacco Cell and Simultaneous Measurement of the Electrical Impedance of Its Cell Membrane

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  • SOTOYAMA Hidekazu
    Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
  • SAITO Mikako
    Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
  • OH Ki-Bong
    Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology
  • MATSUOKA Hideaki
    Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology

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<p>The intracellular potentia1 (VICP) and the cell membrane electrical impedance (ZCM) of a cultured tobacco cel1, line BY-2, were measured simultaneously using a multifunctional microelectrode. In the experiment done in this study, VICP corresponded to the cross membrane potentia1 (VCMP) of the cell. When a control potentia1 (VCON) was varied stepwise in a range from −100 ~ +100mV, VCMP changed accordingly without any significant change in |ZCM|. The relationship between VCON and the change in VCMP (ΔVCMP) showed an asymmetrical property; ΔVCMP/VCON for positive VCON was smaller than that for negative VCON.</p>

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  • Electrochemistry

    Electrochemistry 67 (1), 18-21, 1999-01-05

    公益社団法人　電気化学会

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