Cholinesterase-based Biosensor Using Surface Photovoltage Technique

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  • FEDOSSEEVA Olga V.
    Department of Functional Materials Science, Faculty of Engineering, Saitama University
  • UCHIDA Hidekazu
    Department of Information and Computer Sciences, Faculty of Engineering, Saitama University
  • KATSUBE Teruaki
    Department of Information and Computer Sciences, Faculty of Engineering, Saitama University
  • ISHIMARU Yoshihiro
    Department of Functional Materials Science, Faculty of Engineering, Saitama University
  • IIDA Takeaki
    Department of Functional Materials Science, Faculty of Engineering, Saitama University

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  • Cholinesterase-based Biosensor Using Surface Photovoltage Technique(E)

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Abstract

<p>The new measurement method of Surface Photovoltage (SPV) technique was applied to the fabrication of a novel type cholinesterase-based biosensor. Cholinesterases (butyrylcholine esterase (BuChE) and acetylcholine esterase (AChE)) have been immobilized directly onto the semiconductor surface to measure the concentration of enzyme substrates, via pH changes. The detection limits of the substrates were 9.0×10−7 M, 2.7×10−6 M and 4.1×10−6 M for butyrylthiocholine iodide, acetylcholine iodide and acetylcholine chloride, respectively. The analytical possibilities were examined from the results of the inhibiting actions exerted in the presence of alkaloids such as physostigmine sulfate and neostigmine bromide on BuChE.</p>

Journal

  • Electrochemistry

    Electrochemistry 67 (7), 755-759, 1999-07-05

    The Electrochemical Society of Japan

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