動的光散乱法を用いたタンパク質の結晶化の支援法 Supporting Strategies of Protein Crystallization by Dynamic Light Scattering
The purpose of this technical guide is to describe how dynamic light scattering (DLS) is employed to screen crystallization candidates. Many findings suggest that DLS is useful to evaluate crystallizability of macromolecules before any crystallization attempt. The description is started with an overview of the relation between the crystallizability of macromolecular solution and its monodispersity provided by the DLS measurement. A practical guide for the DLS experiment is also described briefly in the case of using a compact commercial product, Dyna-Pro form Protein Solutions, Inc. Finally, the case study we have done in some protein samples is presented.
日本結晶学会誌 39(4), 315-319, 1997-08-28
The Crystallographic Society of Japan