動的光散乱法を用いたタンパク質の結晶化の支援法

• 加藤 博章

  京都大学化学研究所

書誌事項

タイトル別名

• Supporting Strategies of Protein Crystallization by Dynamic Light Scattering.
• ドウテキ ヒカリ サンランホウ オ モチイタ タンパクシツ ノ ケッショウカ

抄録

The purpose of this technical guide is to describe how dynamic light scattering (DLS) is employed to screen crystallization candidates. Many findings suggest that DLS is useful to evaluate crystallizability of macromolecules before any crystallization attempt. The description is started with an overview of the relation between the crystallizability of macromolecular solution and its monodispersity provided by the DLS measurement. A practical guide for the DLS experiment is also described briefly in the case of using a compact commercial product, Dyna-Pro form Protein Solutions, Inc. Finally, the case study we have done in some protein samples is presented.

収録刊行物

• 日本結晶学会誌

  日本結晶学会誌 39 (4), 315-319, 1997

  日本結晶学会

詳細情報

• CRID: 1390282679064044288
• NII論文ID: 10002589871
• NII書誌ID: AN00188364
• DOI: 10.5940/jcrsj.39.315
• ISSN: 18845576
• ISSN: 03694585
• NDL書誌ID: 4292562
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4292562
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可