Recent Advance in Structural Studies under Extreme Conditions. Rapid Data Collection of Protein Crystals with Laue Diffraction Method.

• ADACHI Shin-ichi

  Biophysical Chemistry Laboratory, The Institute of Physical and Chemical Research (Riken) Harima Institute

Bibliographic Information

Other Title

• 特殊条件下の構造研究の新展開　　白色ラウエ法によるたん白結晶の迅速測定
• ハクショク ラウエホウ ニ ヨル タンパク ケッショウ ノ ジンソク ソクテイ

Abstract

With the use of extremely intense synchrotron radiation sources it has become possible to collect good quality x-ray diffraction data for protein crystals on a subsecond time-scale. This has led to the development of time-resolved protein crystallography, where the main objective is to determine the structures of short-lived intermediates during enzymatic reactions by monitoring the time dependence of the x-ray intensities. Furthermore, utilization of singlebunch mode at the third generation synchrotron sources enables us to shorten the time-scale of the time-resolved protein crystallography down to nanosecond order. Feasibility of singlepulse Laue diffraction experiment on protein crystals is discussed.

Journal

• Nihon Kessho Gakkaishi

  Nihon Kessho Gakkaishi 41 (1), 92-97, 1999

  The Crystallographic Society of Japan

Details

• CRID: 1390282679063094528
• NII Article ID: 10002591538
• NII Book ID: AN00188364
• DOI: 10.5940/jcrsj.41.92
• ISSN: 18845576
• ISSN: 03694585
• NDL BIB ID: 4672643
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4672643
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed