Strand Ligation in a Double-standard DNA by T4 RNA Ligase [in Japanese]
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T4 RNA ligase was shown to ligate two DNA strands contained in a double helix with a high efficiency. Experiments on substrate and temperature dependence of this reaction supported that single-stranded ends clamped by a double-stranded region are necessary for this reaction. This finding is widely useful for DNA joining.
- Chemistry Letters
Chemistry Letters 1996(9), 797-798, 1996-09
The Chemical Society of Japan