Effects of Hydrostatic Pressure on DNA Minor Groove Binding as Studied by Restriction Endonuclease Protection Assay(REPA)
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The effect of pressure on the interaction of minor groove binders with the pBR322 DNA was investigated by using the restriction endonuclease protection assay. The pressure employed alone did not provide measurable enzyme protection whereas it improved the DNA protection ability of drugs. Hydration change terms were proposed to be responsible for the observed pressure effect.
- Chemistry Letters
Chemistry Letters 1997(8), 747-748, 1997-08
The Chemical Society of Japan