Compressibility and Volume Changes of Lysozyme Due to Guanidine Hydrochloride Denaturation
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The apparent specific volume and adiabatic compressibility of lysozyme largely decreased upon guanidine hydrochloride denaturation. These results demonstrate that a large amount of amino acid residues exposes accompanying an increase in hydration and a decrease in the internal cavity. Both parameters deviated from the two-state transition curves, suggesting noncooperative disruption of the compactness and the secondary structure.
- Chemistry Letters
Chemistry Letters 1997(10), 1063-1064, 1997-10
The Chemical Society of Japan