A Novel Fluorogenic Substrate for Horseradish Peroxidase : Efficient Detection of Membrane-Bound Nucleic Acids and Simultaneous Detection of DNAs
Aniline derivatives were found to be trasformed to fluorescent compounds on treatment with horseradish peroxidase/H<SUB>2</SUB>O<SUB>2</SUB>. DNA was detectable with high resolution in the horseradish peroxidase-linked immunosobent fluorescence assay of the membrane-bound DNA using one of the aniline derivatives. A new method for a simultaneous detection of two different DNA sequences was also achieved.
- Chemistry letters
Chemistry letters 1997(10), 1075-1076, 1997-10
The Chemical Society of Japan