Design and Characterization of Polymer Matrix Anchored to Gold Thin Layer for the Effective Usage of Evanescent Field in SPR-based Immunosensor

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A remarkably stable antigen immobilization on gold thin layer deposited on a glass using aminoethanethiol and oxidized dextran hydrosulfate matrix is reported. The polymer was thus covalently bound onto the gold thin layer, and human serum albumin (HSA) as an antigen was covalently bound on the polymer matrix. The association and the dissociation of anti-HSA (aHSA) with the bound HSA were repeatedly observed by surface plasmon resonance (SPR) measurement. The SPR signal vs. concentration of aHSA has a good experimental agreement with the Langmuir adsorption isotherm equation of the aHSA binding with HSA.

収録刊行物

  • Chemistry letters

    Chemistry letters 1997(10), 1083-1084, 1997-10

    The Chemical Society of Japan

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各種コード

  • NII論文ID(NAID)
    10002616283
  • NII書誌ID(NCID)
    AA00603318
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    03667022
  • データ提供元
    CJP書誌  J-STAGE 
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