フィブリンゲル形成の速度論的研究 -フィブリノペプタイドA, B加水分解の意義- Kinetic study on the formation of fibrin gels ; A role of hydrolysis of fibrinopeptides A and B from fibrinogen on gel structure

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著者

    • 佐藤 弘子 SATO Hiroko
    • 京都大学大学院工学研究科高分子化学専攻 Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University
    • 下平 哲司 SHIMOHIRA Tetsuji
    • 京都大学大学院工学研究科高分子化学専攻 Department of Polymer Chemistry, Graduate School of Engineering, Kyoto University
    • 松本 孝芳 MATSUMOTO Takayoshi
    • 京都大学大学院農学研究科森林科学専攻 Devision of Forest and Biomaterial Science, Graduate School of Agriculture, Kyoto University

抄録

Fibrinogen, treated by thrombin, leads to des-AABB-fibrinogen, while Reptilase-treated fibrinogen leads to des-AA-fibrinogen, where fibrinopeptides A and B are abbreviated as A and B, respectively. Both des-AABB-fibrinogen and des-AA-fibrinogen automatically form gels, abbreviated as fnT and fnR, repectively. Their gel structures, however, are difficult to be distinguished by the electron microscopy. For the purpose to compare the gel formation of both des-AABB-fibrinogen and des-AA-fibrinogen, the kinetic change of the absolute values of complex elasticity,|G*|, in the fibrinogen-fibrin (F-fn) conversion to fnT and fnR was investigated in experimental conditions similar to the physiological ones. Moreover, our rheological study was carried out in conditions such as almost the same turbidimetrical changes were observed between F-fnT and F-fnR in the early stage.<BR>There was observed the most remarkable turbidimetrical change subsequently to an induction period. Then,|G*|was found out to increase rapidly, and approached gradually to a maximum value|G*|<SUB>max</SUB>. In fnT,|G*|<SUB>max</SUB> was much higher than that of fnR. The density of cross-linking in fnT was estimated to be higher by 2.25 times than that in fnR from the plots of|G*|against frequency in quasi-equilibrium state. In addition,|G*|<SUB>max</SUB> of fnR was the same as that of fnT, formed in a half concentration of fibrinogen, indicating that the hydrolysis of fibrinopeptide A against the hydrololysis of both fibrinopeptides A and B contributes to the gel structure approximately in a half ratio. In the logarithmic plots of|G*|against (t-t<SUB>0</SUB>), the values for fnT and fnR were overlayed on a curve in the early stage. That is, the structure of fnT appears to be similar to that of fnR in this stage, taking into consideration the more selective hydrolysis of fibrinopeptide A than fibrinopeptide B from fibrinogen by thrombin in physiological conditions.

収録刊行物

  • 日本バイオレオロジー学会誌(B&R) = Journal of Japanese Society of Biorheology  

    日本バイオレオロジー学会誌(B&R) = Journal of Japanese Society of Biorheology 13(1), 28-34, 1999-03-31 

    特定非営利活動法人 日本バイオレオロジー学会

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各種コード

  • NII論文ID(NAID)
    10002622183
  • NII書誌ID(NCID)
    AN10042423
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09134778
  • NDL 記事登録ID
    4699151
  • NDL 雑誌分類
    ZR2(科学技術--生物学--生化学)
  • NDL 請求記号
    Z18-1996
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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