動物細胞潅流培養の温度特性 Temperature Characteristics of Mammalian Cell Perfusion Culture

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著者

    • 高橋 一成 TAKAHASHI Kazunari
    • 東京大学大学院工学系研究科化学生命工学専攻 Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
    • 上田 宏 UEDA Hiroshi
    • 東京大学大学院工学系研究科化学生命工学専攻 Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
    • 鈴木 栄二 SUZUKI Eiji
    • 東京大学大学院工学系研究科化学生命工学専攻 Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

抄録

In the perfusion culture of mammalian cells, the temperature <I>T</I><I><SUB>v</SUB></I> that maximized the viable cell density at steady state was lower by 6-8°C conpared to temperature<I>T</I><I><SUB>g</SUB></I> that maximized cell growth rate. The cell density was 2.4 times as high at <I>T</I><I><SUB>v</SUB></I> as at <I>T</I><I><SUB>g</SUB></I>. Since the death rate decreased as the temperature was lowered, the difference between the growth and death rates could be large enough to maximize the viable cell density, the difference integrated with culture time. Protein production rate per cell as well as per culture volume was maximized at a temperature several degree higher than <I>T</I><I><SUB>v</SUB></I>.

収録刊行物

  • 化学工学論文集  

    化学工学論文集 22(2), 404-407, 1996-03-10 

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10002668522
  • NII書誌ID(NCID)
    AN00037234
  • 本文言語コード
    JPN
  • 資料種別
    NOT
  • ISSN
    0386216X
  • NDL 記事登録ID
    3937005
  • NDL 雑誌分類
    ZP5(科学技術--化学・化学工業--化学工学)
  • NDL 請求記号
    Z17-725
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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