High Density Culture of Streptococcus lactis 527 Using Electro-microfiltration Bioreactor.

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  • Hakoda Masaru
    Department of Biological and Chemical Engineering, Gunma University
  • Enomoto Atsushi
    Department of Biological and Chemical Engineering, Gunma University
  • Nakamura Kozo
    Department of Biological and Chemical Engineering, Gunma University Division of Agricultual and Agricultual Life Sciences, The University of Tokyo

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  • 電気精密ろ過反応器を用いたＳｔｒｅｐｔｏｃｏｃｃｕｓ　ｌａｃｔｉｓ　５２７の高濃度培養
  • デンキ セイミツ ロカ ハンノウキ オ モチイタ Streptococcus

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Abstract

Electro-microfiltration bioreactor (EMFBR) was applied to the high density culture of Streptococcus lactis 527, and the effect of electric field on the filtrate flux and the cell concentration was examined experimentally.<BR>The EMFBR allowed complete retention of cells in the fermentor and continuous removal of inhibitory metabolizes. S. lactis was grown in the EMFBR with the maximum concentration of 24.2 g-dry cells/ l, about 10 times as much as that of the conventional batch culture, and this improvement was due to removal of lactic acid from the fermentor. The yield of cell against glucose YX/S and the maintenance coefficient m both increased by application of an electric field. The experimental results showed that EMFBR could be effective if the experimental conditions were chosen properly.

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