温度誘導型遺伝子組換え大腸菌培養における遺伝的アルゴリズムを利用した最適培養温度・ｐＨ変化パターン

• 五十嵐 久人

  九州工業大学情報工学部 生物化学システム工学科

• 中川 秀人

  九州工業大学情報工学部 生物化学システム工学科

• 清水 和幸

  九州工業大学情報工学部 生物化学システム工学科

書誌事項

タイトル別名

• Optimal Temperature and pH Pattern for the Cultivation of Temperature Inducible Gene Engineered Escherichia coli Utilizing Genetic Algorithm.
• オンド ユウドウガタ イデンシ クミカエ ダイチョウキン バイヨウ ニ オケル

抄録

Fed-batch cultivation of gene-engineered E. coli was conducted using 1.4 dm³ scale fermenter, where plasmid pRZ, which contained A-phage P_R promoter and lacZ structure gene, was used to transform the Escherichia coli N 4830-1. Two stage cultivation method was employed, where temperature and pH were controlled at 30°C and 7.0, respectively, during the initial 8 hours to enhance the cell growth. After 8 hours, the culture temperature was raised to 37-42°C to induce gene expression, where pH was also changed for the efficient production of gene product (β-galactosidase).<BR>Based on the results obtained from several fed-batch runs of cultivations, an unstructure model was developed to derive the optimal temperature and pH patterns using a Genetic Algorithm. Experiments were conducted based on the derived temperature and pH patterns, and it was verified that the productivity of gene product was the highest among the past experiments.

収録刊行物

• 化学工学論文集

  化学工学論文集 22 (6), 1391-1399, 1996

  公益社団法人 化学工学会

キーワード

• Gene-Engineered E. coli
• Optimal Operation
• PR Promoter
• β-Galactosidase
• Genetic Algorithm

詳細情報

• CRID: 1390282679485245184
• NII論文ID: 10002669933
• NII書誌ID: AN00037234
• DOI: 10.1252/kakoronbunshu.22.1391
• ISSN: 13499203
• ISSN: 0386216X
• NDL書誌ID: 4078158
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4078158
• Web Site: http://www.jstage.jst.go.jp/article/kakoronbunshu1975/22/6/22_6_1391/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可