Production of Functional Compost Aimed at Repressing the Lawn Pathogen through the Lnoculation of <I>Bacillus Subtilis</I> into Mowed Lawn

• Nakasaki Kiyohiko

  Department of Materials Science & Chemical Engineering, Faculty of Engineering, Shizuoka University

• Kishiro Yoshiyuki

  Department of Materials Science & Chemical Engineering, Faculty of Engineering, Shizuoka University

• Hiraoka Sachiko

  Department of Materials Science & Chemical Engineering, Faculty of Engineering, Shizuoka University

Bibliographic Information

Other Title

• <I>Bacillus subtilis</I>を接種した芝草病害防除コンポストの作成
• Bacillus subtilis オ セッシュシタ シバクサ ビョウガイ ボ

Abstract

Inoculation of suppressive bacteria into compost raw material was carried out in order to make a functional compost aimed at repressing lawn disease. A spontaneous streptomycin-resistant mutant of suppressive bacterium, B. subtilis N 4- 1 was used to investigate the dynamic change of the inoculated strain during composting. It was concluded that it is important to keep the concentration of the bacteria indigenous in the raw material as low as possible in order to enable the growth of N 4-1. The compost product made from the raw material containing other bacteria less than 4.3 × 10⁵ CFU/g-ds showed a suppressive effect on the pathogen. The feasibility of repeatedly using the compost product as a seed for the next composting was also examined. The concentration of N 4-1, and the ratio of N 4-1 to total bacteria (estimated by summing up the N 4-1 and the other bacteria) in the compost product decreased rapidly as the number of repetitions was increased. The compost product lost the suppressive effect after the 5 th repetition.<BR>It was also ascertained that the ratio of N 4-1 to total bacteria had a strong effect on existence and degree of suppressiveness. The suppressive test experiment using cell suspension mixtures of pure cultured N 4-1 and insuppressible bacterium, Escherichia coli E 2 was performed to ascertain this.

Journal

• Journal of the Japan Society of Waste Management Experts

  Journal of the Japan Society of Waste Management Experts 8 (4), 166-174, 1997

  Japan Society of Material Cycles and Waste Management

Keywords

• composting
• inoculum
• plant disease
• biological control
• <I>Bacillus subtilis</I>

Details

• CRID: 1390001205296833280
• NII Article ID: 10002676249
• NII Book ID: AN10414685
• DOI: 10.3985/jswme.8.166
• ISSN: 1883163X
• ISSN: 18831648
• NDL BIB ID: 4237332
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4237332
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed