Vacuolar Acidification in Saccharomyces cells in Response to Elevated Hydrostatic Pressure.

• Abe F.

  The DEEPSTAR group, Japan Marine Science and Technology Center

• Horikoshi K.

  The DEEPSTAR group, Japan Marine Science and Technology Center

Bibliographic Information

Other Title

• Vacuolar Acidification in Saccharomyces

Abstract

A pressure of 40-60MPa promoted the acidification of the yeast vacuoles. The pressure-induced acidification was observed in the presence of fermentable sugars such as D-glucose and was inhibited by 2-deoxy-D-glucose, suggesting that CO₂ generated through ethanol fermentation was involved in the process. The concentration of glucose required for half maximal changes in pressure-induced acidification was approximately 21mM, which was close to apparent Km for the low affinity component of the glucose transporter on the plasma membrane (∼20mM). It is likely that glucose transport may be a rate-limiting step in the overall kinetics of vacuolar acidification induced by elevated hydrostatic pressure.

Journal

• The Review of High Pressure Science and Technology

  The Review of High Pressure Science and Technology 7 1312-1314, 1998

  The Japan Society of High Pressure Science and Technology

Keywords

• yeast vacuole
• vacuolar acidification
• fluorescence analysis
• chemical reaction of CO<SUB>2</SUB>
• Barophysiology

Details

• CRID: 1390282679357237504
• NII Article ID: 10002693900
• NII Book ID: AN10452913
• DOI: 10.4131/jshpreview.7.1312
• ISSN: 13481940
• ISSN: 0917639X
• NDL BIB ID: 4494224
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4494224
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed