Analysis of the Upstream Element of the Pressure-regulated Operon from Deep-sea Barotolerant Shewanella sp. DSS12

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Upstream element of Pressure-regulated operon from a deep-sea barotolerant bacterium strain DSS12 under several pressures was studied. Region A and region B were classified by sequence analysis. A unique octamer motif AAGGTAAG, was found to be tandemly repeated 13 times in Region B. Electrophoretic mobility shift assay demonstrated that σ<SUP>54</SUP>-like factor recognized Region A and another factors recognized Region B of the operon. Different shift patterns of the protein-DNA complexes were observed when cell extracts cultured at 0. 1MPa and 50MPa were incubated with the DNA probe of Region B. These results indicate that the strain expresses different DNA-binding factors under different pressure conditions.

収録刊行物

  • 高圧力の科学と技術 = The Review of high pressure science and technology  

    高圧力の科学と技術 = The Review of high pressure science and technology 7, 1318-1320, 1998 

    The Japan Society of High Pressure Science and Technology

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各種コード

  • NII論文ID(NAID)
    10002693936
  • NII書誌ID(NCID)
    AN10452913
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    0917639X
  • NDL 記事登録ID
    4494226
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z17-1589
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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