オキシランアクリル粒子固定化酸化還元酵素充填カラムを用いた基質の分析 [in Japanese] Analysis of Substrate Concentratin Using Immobilized Oxidoreductase Columns Packed with Oxirane Acrylic Particle as Supporting Material [in Japanese]
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Catalase, ascorbate oxidase, glucose oxidase and uricase immobilized on oxirane acrylic particles were packed into a column. Each sample of substrate solution of volume <I>G</I> and substrate concentration <I>C</I> was injected into the inlet flow of the fixed buffer solution flowing through the column at the rate <I>v</I> and temperature T, and the dissolved oxygen concentration in the outlet flow was analyzed by a dissolved oxygen sensor. Sensor output was integrated with respect to time to obtain S. In each sample, stoichiometric relations of <I>S</I> were formed approximately in under about <I>S</I> = 5.5 mv·min.
- Chemical engineering
Chemical engineering 23(3), 455-459, 1997-05-10
The Society of Chemical Engineers, Japan