高等植物由来キチナーゼの抽出とキチン分解活性 Extraction of Crude Chitinase from Higher Plants and their Chitin-Hydrolysis Activities
To prepare a purified chitinase from higher plants, firstly, crude enzymes were extracted from six higher plants, namely, radish seeds, sunflower seeds, watermelon seeds, bamboo leaves, orange skin, and persimmon skin. Using these crude enzymes, pH dependencies of hydrolysis reaction of colloidal chitin are investigated. For radish seeds and bamboo leaves, which have relatively high activities, the kinetics of enzymatic reaction are studied. It is clear that these reactions obey Michaelis-Menten kinetics.
化学工学論文集 23(4), 601-603, 1997-07-10
The Society of Chemical Engineers, Japan