W/O型エマルションを利用した界面活性剤 -酵素複合体の調製 Preparation of Surfactant-Enzyme Complex Utilizing Water-in-Oil Emulsion
A novel preparation method for surfactant-enzyme complexes applicable in organic media is developed utilizing a water-in-oil (W/O) emulsion. This preparation method entails simple stages while it offers the advantages of enhanced enzymatic activity in the reacting media and eliminates enzyme loss. Using the surfactant-chymotrypsin complex, peptide synthesis with amino acid derivatives was conducted in tetrahydrofuran. The surfactant-chymotrypsin complex indicates higher activity than native chymotrypsin. This modified method will facilitate the usage of enzymes in organic media as new biocatalysts.
化学工学論文集 23(4), 607-609, 1997-07-10
The Society of Chemical Engineers, Japan