Physical Mapping of 5S rRNA Genes in Cycas revoluta.

  • Hizume M.
    Biological Institute, Faculty of Education, Ehime University

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The 5S rRNA gene (5S rDNA) was physically mapped on the somatic chromosomes (2n=22) in Cycas revoluta Thunb. by in situ hybridization using biotin-labeled probes amplified from total genomic DNA. The 5S rDNA was localized at approximate terminal region of the longest pair of telocentric chromosomes. The site of 5S rDNA were the same as C- or chromomycin A3-band.

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