Plant Regeneration from Septum Segment of a Water Plant Pak-bung (Ipomoea aquatica).

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  • Mori Kazuhiro
    Department of Environmental Engineering, Graducate school of Engineering, Osaka University
  • Igehara Hiroyuki
    Department of Environmental Engineering, Graducate school of Engineering, Osaka University
  • Yoshida Kazuya
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • Shinmyo Atsuhiko
    Graduate School of Biological Sciences, Nara Institute of Science and Technology
  • Fujita Masanori
    Department of Environmental Engineering, Graducate school of Engineering, Osaka University

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Abstract

Pak-bung (Ipomoea aquatica) is a water plant which is useful to the water purification due to the high nutrient-removal capacity. An efficient plant regeneration method from septum segment of pak-bung were established. The septum calli were induced on the modified MS solid (MMS) medium supplemented with 1 mg/l of 1-naphthalene-acetic acid (NAA) and benzyladenine (BA) respectively. Shoots were regenerated from septum calli with various combinations of BA (0-10 mg/l) and NAA (0-1 mg/l) and the highest regeneration frequency (77%) were observed on the medium containing 5 mg/l of BA. Roots were induced successfully from all of the regenerated shoots applied to the rooting test and plantlets were reproduced. There was no morphological difference between regenerated plants and plants grew up from seeds. Using the culture conditions established in this study, the regenerated plant of pak-bung can be produced in a short period (4-6 weeks) .

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