書誌事項
- タイトル別名
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- Establishing an Apoptosis-Resistant Hybridoma Cell Line for Usage in Serum Starvation Culture.
- ケッセイ キガ バイヨウ ノ ジツゲン オ メザシタ ハイブリドーマ サイボウ
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抄録
A serum starvation culture of hybridoma cells is desirable because serum starvation stops cell cycling at the G1 phase, where hybridoma cells produce more antibody in the other phases, However, serum starvation induces apoptosis, a type of cell death, forcing the culture to die out. In this research, the authors aimed at inhibition of cell death during serum starvation culture. A hybridoma cell line bcl-2 was transfected with bcl-2, an apoptosis suppressing gene, and named 2E3-bcl 2. Then the cells were screened in serum starvation culture to obtain 2E3-bcl 2-LS. The expression level of Bcl-2 protein in 2E3-bcl 2-LS was higher by 40% than that in 2E3-bcl-2. In serum starvation culture, 2E3-bcl 2-LS survived longer by two days and produced twice as much antibody as the wild type.
収録刊行物
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- 化学工学論文集
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化学工学論文集 24 (1), 161-164, 1998
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001204508688512
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- NII論文ID
- 10002835112
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- NII書誌ID
- AN00037234
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- ISSN
- 13499203
- 0386216X
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- NDL書誌ID
- 4380965
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可