Establishing an Apoptosis-Resistant Hybridoma Cell Line for Usage in Serum Starvation Culture.

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  • TERADA SATOSHI
    Department of Chemistry and Biotechnology, Graduate School of Engineering, the University of Tokyo
  • ITOH YOHITO
    Department of Chemistry and Biotechnology, Graduate School of Engineering, the University of Tokyo
  • SUZUKI EIJI
    Catalysis Science Lab., Research Institute of Innovative Technology for the Earth

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  • 血清飢餓培養の実現を目指した，ハイブリドーマ細胞への細胞死耐性能の付与
  • ケッセイ キガ バイヨウ ノ ジツゲン オ メザシタ ハイブリドーマ サイボウ

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Abstract

A serum starvation culture of hybridoma cells is desirable because serum starvation stops cell cycling at the G1 phase, where hybridoma cells produce more antibody in the other phases, However, serum starvation induces apoptosis, a type of cell death, forcing the culture to die out. In this research, the authors aimed at inhibition of cell death during serum starvation culture. A hybridoma cell line bcl-2 was transfected with bcl-2, an apoptosis suppressing gene, and named 2E3-bcl 2. Then the cells were screened in serum starvation culture to obtain 2E3-bcl 2-LS. The expression level of Bcl-2 protein in 2E3-bcl 2-LS was higher by 40% than that in 2E3-bcl-2. In serum starvation culture, 2E3-bcl 2-LS survived longer by two days and produced twice as much antibody as the wild type.

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