血清飢餓培養の実現を目指した，ハイブリドーマ細胞への細胞死耐性能の付与

• 寺田 聡

  東京大学大学院工学系研究科化学生命工学専攻

• 伊藤 世人

  東京大学大学院工学系研究科化学生命工学専攻

• 鈴木 栄二

  (財) 地球環境産業技術研究機構環境触媒研究室

書誌事項

タイトル別名

• Establishing an Apoptosis-Resistant Hybridoma Cell Line for Usage in Serum Starvation Culture.
• ケッセイ キガ バイヨウ ノ ジツゲン オ メザシタ ハイブリドーマ サイボウ

抄録

A serum starvation culture of hybridoma cells is desirable because serum starvation stops cell cycling at the G₁ phase, where hybridoma cells produce more antibody in the other phases, However, serum starvation induces apoptosis, a type of cell death, forcing the culture to die out. In this research, the authors aimed at inhibition of cell death during serum starvation culture. A hybridoma cell line bcl-2 was transfected with bcl-2, an apoptosis suppressing gene, and named 2E3-bcl 2. Then the cells were screened in serum starvation culture to obtain 2E3-bcl 2-LS. The expression level of Bcl-2 protein in 2E3-bcl 2-LS was higher by 40% than that in 2E3-bcl-2. In serum starvation culture, 2E3-bcl 2-LS survived longer by two days and produced twice as much antibody as the wild type.

収録刊行物

• 化学工学論文集

  化学工学論文集 24 (1), 161-164, 1998

  公益社団法人 化学工学会

キーワード

• hybridoma
• monoclonal antibody
• apoptosis
• bcl-2
• serum starvation

詳細情報

• CRID: 1390001204508688512
• NII論文ID: 10002835112
• NII書誌ID: AN00037234
• DOI: 10.1252/kakoronbunshu.24.161
• ISSN: 13499203
• ISSN: 0386216X
• NDL書誌ID: 4380965
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4380965
• Web Site: http://www.jstage.jst.go.jp/article/kakoronbunshu1975/24/1/24_1_161/_pdf
• 本文言語コード: ja
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可