書誌事項
- タイトル別名
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- Biomedical Engineering. Three-dimensional Primary Cultures and Characterization of Monolayers with the Rabbit's Tracheal Epithelium.
- ウサギ キカン ジョウヒ ネンマク ノ 3ジゲン ショダイ サイボウ バイヨウ
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Confluent monolayers of epithelial cells from a rabbit's tracheal mucosa is obtained via several methods of primary cultures, and characterization of the monolayers is discussed in terms of morphology and electrical resistance as follows : The tracheal epithelial (TE) cells are isolated with little damage under the condition that the trachea was immersed in the type XIV protease of 0.1%. In the cell culture, a serum-free hormone-supplemented medium is used for the growth of TE cells with cell culture inserts. The cilia and desmosome are observed in the monolayers cultured for 10 days ; the TE cells were seeded on a collagen-coating membrane, and the mucosal side of the monolayers was out of the medium. The data of electrical resistance of monolayers indicates that a suitable duration is 9-10 days for the cell culture to construct a monolayer of the real tracheal epithelium.
収録刊行物
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- 化学工学論文集
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化学工学論文集 24 (2), 238-242, 1998
公益社団法人 化学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001204509651456
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- NII論文ID
- 10002835310
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- NII書誌ID
- AN00037234
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- ISSN
- 13499203
- 0386216X
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- NDL書誌ID
- 4424755
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可