液／液界面における付着依存性動物細胞の増殖および付着に対する界面活性物質の影響

• 芝 良昭

  群馬大学工学部生物化学工学科

• 大嶋 孝之

  群馬大学工学部生物化学工学科

• 佐藤 正之

  群馬大学工学部生物化学工学科

書誌事項

タイトル別名

• Effect of Surface Active Compounds on Growth and Adhesion of Anchorage-Dependent Animal Cells at Liquid/Liquid Interface.
• エキ エキ カイメン ニ オケル フチャク イゾンセイ ドウブツ サイボウ ノ

抄録

As a novel cell cultivation method, cell cultivation using a liquid/liquid interface system (culture medium/fluorocarbon) has been investigated. Cell adhesion at the interface appears to be dependent on the contaminants contained in the fluorocarbon. In this study, the effect of surfactant on the growth and adhesion of the cells was studied. Although the cells could not anchor at the interface between nonionic surfactant-added medium and fluorocarbon, cell growth was observed when ionic surfactant, instead of nonionic surfactant, was used. When ionic surfactant was added to fluorocarbon, cell growth was better than that at the interface between ionic surfactant-added medium and fluorocarbon. In particular, the cell growth at the interface using fluorocarbon added pentafluorobenzoyl chloride or perfluorooctanoyl chloride was similar to that at the polystyrene surface, which indicates that it is important to add the surfactant to fluorocarbon.

収録刊行物

• 化学工学論文集

  化学工学論文集 24 (2), 343-345, 1998

  公益社団法人 化学工学会

キーワード

• anchorage-dependent cell
• liquid/liquid interface
• fluorocarbon
• surfactant
• cell adhesion

詳細情報

• CRID: 1390282679484912768
• NII論文ID: 10002835509
• NII書誌ID: AN00037234
• DOI: 10.1252/kakoronbunshu.24.343
• COI: 1:CAS:528:DyaK1cXhvVGhu78%3D
• ISSN: 13499203
• ISSN: 0386216X
• ISSN: http://id.crossref.org/issn/0386216X
• NDL書誌ID: 4424773
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4424773
• Web Site: http://www.jstage.jst.go.jp/article/kakoronbunshu1975/24/2/24_2_343/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可